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Headspace Vials. A headspace vials is used for creating a headspace sample within the vial. By introducing the sample into the vial the sample diffuses to gas. Photograph showing the experimental set up. 20 mL glass . 20 mL glass headspace vials were filled with 1 g of seeds (seeds pictured are Lolium perenne) which had been equilibrated to 60% RH.

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