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2023年1月26日 · There are four steps in the ammonium sulfate precipitation procedure: Fractionation of ammonium sulfate, treatment with cooled acetone and hexane, and chromatography on a silica gel column. At 4 °C, centrifuge the culture broth at 10,000 rpm for 15 min. Take the supernatants and precipitate them with 40% (NH 4 )SO 4.
